Влияние индол-3-масляной кислоты и хлормеквата хлорида на рост растений томата

Relevance. This study aimed to improve fruit set and plant performance to increase tomato productivity by studying the effect of plant growth regulators on tomato plants.Methods. A specific experiment has been carried out to study the effect of plant growth regulators Milagro (1% indol-3-butric acid) and Atlet active substances indol-3-butric acid and chloromequate chloride with applied doses (0.6 M/L, 1.0 M/L and 1.5 M/L) and (1.0, 1.5 and 2.0 M/L) on growth and physiological characteristics of plants (Big Beef F1). The experimental design was a Complete Randomized Blocks Design. Both Hemo bles was applied three times (spraying on plants at 30 DAP, spraying on plants at 60 DAP and spraying on plants 90 DAP).Results. The obtained results showed that, Applying Milagro (1% indol-3-butric acid) had the highest significant Plant height (80.13, 128.77 and 239 cm), number of leaves/plant (18.0, 34.67 and 44.3) and stem diameter (1.07, 1.5 and 2.03 cm), fruit weight (122.0 and 136 g), Flower Clusters number in the plant (4.64, 13.33 and 16.33) and Fruits Number (61.67, 62.0 and 67) Over the three years of study. The results were analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s HSD test with α = 0.05 with the help of MINITAB (v. 19.0) program.Актуальность. Исследование было направлено на улучшение плодоношения и продуктивности растений для увеличения урожайности томатов путем изучения влияния регуляторов роста растений.Материал и методы. Проведен эксперимент по изучению влияния регуляторов роста растений Милагро (1% индол-3-масляная кислота) и активных веществ Атлет – индол-3-масляной кислоты и хлормеквата хлорида, применяемых в дозах (0,6 м/л, 1,0 м/л) и 1,5 м/л) и (1,0, 1,5 и 2,0 м/л) по росту и физиологическим характеристикам растений (Big Beef F1). Оба регулятора роста наносили три раза (опрыскивание растений при 30 DAP, опрыскивание растений при 60 DAP и опрыскивание растений при 90 DAP).Результаты. Применение Милагро (1% индол-3-масляной кислоты) оказало наибольшее влияние на высоту растений (80,13, 128,77 и 239 см), количество листьев / растений (18,0, 34,67 и 44,3) и диаметр стебля (1,07, 1,5 и 2,03 см), массу плодов (122,0 и 136 г), количество цветочных кистей на растении (4,64, 13,33 и 16,33) и количество плодов (61,67, 62,0 и 67) в течение трех лет исследования. Результаты были проанализированы с использованием одностороннего дисперсионного анализа (ANOVA) с последующим HSD-тестом Тьюки с α = 0,05 с помощью программы MINITAB (v. 19,0)

Measurements of the normal size of spleen and spleen to left kidney ratio among sudanese children up to five years age using ultrasonography

Background: Knowledge of the normal range of spleen size in the population being examined is very important because there are many different pathological conditions that may alter in spleen size. Also, gender, height, weight and BMI of individual, affect the splenic length and could result in incorrect interpretation of splenic measurements, spleen to left kidney ratio is one of recent parameter mention in literature aid in diagnosis of splenomegaly in children.Methods: The study was a cross-sectional study conducted in Khartoum state at Al-Buluk and Gaafar Ibnauf Paediatric Tertiary Hospital during the period from March 2018 to August 2018. A total of 101 children (males and females) with aged ranged 1 month to 5 years included in study. A transabdominal scan was performed, and measurement of spleen and left kidney taken after an ethical verbal consent from their parents, then data analyzed by SPSS, Mean±SD. Deviation for study variables calculated then correlation between patients factors and ultrasound measurements done.Results: The study found that the mean measurements of the normal size of the spleen and spleen to left kidney ratio for children up to five years of age, Splenic length was 7.24±1.12 cm, width was 3.83±0.81 cm , thickness 2.91±0.55 cm and spleen to left kidney ratio was 1.04±0.0.70 ranged (0.86-1.23). The male have larger spleen than female with no statistically significant differences in spleen sizes between the sexes. There was a strong significant correlation between age, weight, height  spleen and left kidney length (p<0.01)respectively and a moderate association with BMI (p<0.05).Conclusions: Spleen and left kidneys was growing rapidly and similarly in infant up to one year of life 1 mm\1 mm for each month and then decreasing growth rate of kidney in relate to spleen for 1 mm growth of spleen kidney grow 0.8 mm in up to five years of age. There was strong significant positive correlation between spleen and left kidney measurement with age, height, weight of individual. The spleen to left kidney ratio for children up to five years ranged (0.86-1.23) with mean 1.04, so if the ratio less or more than this ranged it should be considered in clinical context for diagnosis of splenomegaly or shrunken spleen in children up to five years of age

Doppler ultrasound of umbilical artery in prediction of fetal outcome in pregnancy induced hypertension Sudanese population

The pregnancy induced hypertension increase the fetal mortality and morbidity and the using of Doppler umbilical artery indices decrease the fetal mortality and morbidity however, there is few complete data about the most frequently altered Doppler US parameters to predict fetal outcome in pregnancy induced hypertension . Methods This ia cohort prospective study done in two hundred and six women of second and third trimester presenting to antenatal clinic in Soba University Hospital at the department of Obstetrics & Gynecology, in the fetus unit and critical pregnancy in the period From June 2008 to April 2013 to assess the Doppler indices of umbilical artery in pregnancy induced hypertension for prediction of prenatal outcome; 105 pregnancy induced hypertension patients and 101 women with uneventful pregnancies as normal control group included in this study . Baseline investigations and color Doppler of umbilical artery were done. Statistical analysis of data were done using SPSS, Receiver Operating Characteristic (ROC) curve analysis was performed and the area under the curve (AUC) used to determine sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of umbilical artery Doppler indices to predict fetal outcome.Results The study determine that there was significant difference in Doppler indices in PIH and control group ( p<0.01, the mean indices of umbilical artery is higher in PIH group compared with normal pregnancy group , the mean different of S/D ratio was 0.40, mean difference of RI was 0.06 and the mean different of PI index was 0.16, high percentage of adverse fetal outcome had been reported in in Pregnancy Induced Hypertension group than in control , which was more in absent and reversed flow velocity in umbilical artery in Pregnancy Induced Hypertension compared with group of Pregnancy Induced Hypertension with present end diastolic flow velocity. Systolic/Diastolic ratio was most accurate in predicting adverse outcome in pregnancy induced hypertension patients, followed by the Pulastility index then the Resistance index (75%, 66% and 57% respectively).ConclusionThis study concluded that pregnancy induced hypertension leads to worsen placental insufficiently, which appears on the higher Doppler indices of umbilical artery to PIH patients when compared with normal pregnancy. A low diastolic flow and higher indices characterized the pregnancies with abnormal outcomes. Doppler of the umbilical artery was useful to predict fetal well being in PIH patients, high percentage of adverse fetal outcome had been reported in absent and reversed end diastolic flow velocity in umbilical artery compared with group of present flow velocity

Photonuclear spectroscopy with the ELIADE array at ELI-NP

The Extreme Light Infrastructure – Nuclear Physics in BucharestM˘agurele, Romania, is a major European undertaking with the aim of constructing a facility that can produce the worlds highest intensity laser beams as well as unique high-brilliance, narrow-bandwidth gamma-ray beams using laser-based inverse Compton scattering. One of the main instruments being constructed for the nuclear physics and applications with high-brilliance gamma-beams research activity is the ELIADE detector array of eight segmented HPGe clover detectors. Using the nuclear resonance fluorescence technique this setup will provide us with access to several nuclear observables like spins, parities, level widths, and branching ratios in the decay. From these observables we expect to draw conclusions about, for example, nuclear dipole response, properties of pygmy resonance and collective scissors mode excitations, parity violation in nuclear excitations, and matrix elements for neutrinoless double-beta decay, among other topics. The uniqueness of the environment in which ELIADE will operate presents several challenges in the design and construction of the array. In this contribution we will present some of these challenges and how these challenges are overcome

Measurement of normal optic nerve for Sudanese pediatric using magnetic resonance imaging

Background: Much congenital and intracranial pathology may affect optic nerve (ON) and caused increased or decreased in size, so the measurement is crucial and aiding in diagnosed of some neurogenic and endocrine disorders. The aim of this study was to measure the normal ON length and diameter by magnetic resonance imaging among pediatric at Khartoum state Sudan.Methods: This was descriptive, cross section study,  done in 100 Sudanese pediatrics with age from 1 month to 15 years came to Magnetic resonance imaging (MRI) department for MRI brain at three hospitals in Khartoum state  (Al-Amal national hospital, Al-Zaitona hospital and Modern Medical Center) during the period from December 2019 to March 2020, all of patients had no pathological or medical condition that may affect the orbits and optic nerve (OON), any child with and medical condition or pathology related to orbits excluded from study sampling. The data were collected by data collection sheet designed especially for this study then analyzed.Results: The study found that the mean diameter of ON was 2.06±0.44 mm, the mean length of right ON was 31.54±4.11 mm, there is no significant different in measurement between males and females (p>0.05), there was strong significant correlation between ON length and diameter with children age (r=0.592**, 0.654**, p<0.001 respectively).Conclusions: The study concluded that there was strong correlation between right and left ON diameter and length with age

Simulation Study on Computer Tomography Imaging of Nuclear Distribution by Quasi Monoenergetic Gamma Rays with Nuclear Resonance Fluorescence: case study for ELI-NP application

CoE on Sustainable Energy System (Thai-Japan), Faculty of Engineering, Rajamangala University of Technology Thanyaburi (RMUTT), ThailandNon-destructive inspection carried out by using nuclear resonances excited by an MeV energy region gamma ray is a promising method. The high penetrability of MeV gamma ray of nuclear resonant energy makes possible the detection of nuclides surrounded by massive materials. As an application of this method, computed tomography imaging of nuclear distribution inside objects can be reconstructed from transmission factor of gamma rays. We have studied the image reconstruction of the nuclear distribution using Monte-Carlo simulations to estimate the gamma-ray transmission factor assuming the ELI-NP facility where about 3 order higher intensity of quasi-monoenergetic gamma rays will be available

Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes

Introduction: In recent years, there has been an exponential increase in the number of studies aiming to understand the biology of exosomes, as well as other extracellular vesicles. However, classification of membrane vesicles and the appropriate protocols for their isolation are still under intense discussion and investigation. When isolating vesicles, it is crucial to use systems that are able to separate them, to avoid cross-contamination. Method: EVs released from three different kinds of cell lines: HMC-1, TF-1 and BV-2 were isolated using two centrifugation-based protocols. In protocol 1, apoptotic bodies were collected at 2,000&#x00D7;g, followed by filtering the supernatant through 0.8 &#x00B5;m pores and pelleting of microvesicles at 12,200&#x00D7;g. In protocol 2, apoptotic bodies and microvesicles were collected together at 16,500&#x00D7;g, followed by filtering of the supernatant through 0.2 &#x00B5;m pores and pelleting of exosomes at 120,000&#x00D7;g. Extracellular vesicles were analyzed by transmission electron microscopy, flow cytometry and the RNA profiles were investigated using a Bioanalyzer&#x00AE;. Results: RNA profiles showed that ribosomal RNA was primary detectable in apoptotic bodies and smaller RNAs without prominent ribosomal RNA peaks in exosomes. In contrast, microvesicles contained little or no RNA except for microvesicles collected from TF-1 cell cultures. The different vesicle pellets showed highly different distribution of size, shape and electron density with typical apoptotic body, microvesicle and exosome characteristics when analyzed by transmission electron microscopy. Flow cytometry revealed the presence of CD63 and CD81 in all vesicles investigated, as well as CD9 except in the TF-1-derived vesicles, as these cells do not express CD9. Conclusions: Our results demonstrate that centrifugation-based protocols are simple and fast systems to distinguish subpopulations of extracellular vesicles. Different vesicles show different RNA profiles and morphological characteristics, but they are indistinguishable using CD63-coated beads for flow cytometry analysis

PCR array and protein array studies demonstrate that IL-1β (interleukin-1β) stimulates the expression and secretion of multiple cytokines and chemokines in human adipocytes

The role of IL-1β in regulating the expression and secretion of cytokines and chemokines by human adipocytes was examined. Adipocytes were incubated with human IL-1β for 4 or 24 h. The expression of a panel of 84 cytokine/chemokine genes was probed using PCR arrays. IL-1β stimulated the expression of >30 cytokine/chemokine genes on the arrays; 15 showed >100-fold increases in mRNA at 4 or 24 h including CSF3, CXCL1, CXCL2, CXCL12 and IL8. CSF3 exhibited a 10,000-fold increase in mRNA at 4 h. ADIPOQ was among the genes whose expression was inhibited. Protein arrays were used to examine the secretion of cytokines/chemokines from adipocytes. IL-1β stimulated the secretion of multiple cytokines/chemokines including MCP-1, IL-8, IP-10, MIP-1α and MCP-4. The most responsive was IP-10, which exhibited a 5,000-fold increase in secretion with IL-1β. IL-1β is likely to play a substantial role in stimulating the inflammatory response in human adipocytes in obesity

Влияние регуляторов роста на качество плодов томата

Relevance and methods. This study aimed to improve fruit set and plant performance to increase tomato productivity by studying the effect of plant growth regulators on tomato plants. A specific experiment has been carried out to study the effect of plant growth regulators Hemo bles active substances (850 g/kg) Humic Acid with applied doses (250, 500 and 700 ppm) and Magictone active substances (5-12.5 g/kg) naphthalene acetic acid and naphthalene acetamide with applied doses (250, 500 and 700 ppm) on growth and physiological characteristics of tomato plants (Big Beef F1). The experimental design was a Complete Randomized Blocks Design. Both Hemo bles and Magictone were applied three times (spraying on plants at 30 DAP, spraying on plants at 60 DAP and spraying on plants 90 DAP).Results. The obtained results showed that, Applying Humic Acid “Ener-850” had the highest significant fruit weight (137 g) during the two seasons. Also using “Magictone” had the highest significant Flowers number (48.1), Fruits Number (35.1), Flower Clusters number in the plant (13.6) and Fruits Number (54.6. while (Humic Acid) improved tomato fruit’s quality during improve Dry weight (75.1 g) of Arial parts, Ascorbic Acid, level of Vitamin C and Carotenoids contents (4.82 mg 100 g-1). The results were analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s HSD test with α = 0.05 with the help of MINITAB (v. 19.0) program.Актуальность и методика. Наши исследования были направлены на изучение влияния регуляторов роста Hemo bles и Magictone на продуктивность томата Биг Биф F1 (Big Beef F1). Гибрид среднеспелый, индетерминантного типа. Обработку проводили 3 раза с интервалом в 30 дней, концентрация растворов – 250 мг/л, 500 мг/л, 700 мг/л.Результаты. В результате исследований установлено, что применение Hemo bles (гуминовая кислота, 850 г/кг) и Magictone (нафталин уксусная кислота и нафталин ацетамид, 5-12,5 г/кг) увеличивает показатели продуктивности растений томата. При обработке Magictone наблюдали наибольшее количество плодов на растении – 63,7 шт., количество кистей 14,2 шт. и массу одного плода – 144,0 г, в то время как Hemo bles оказывал наибольшее влияние на показатели: содержание сухого вещества до 7,65%, содержание аскорбиновой кислоты – до 27,0 мг/100 г и каротиноидов – 4,92 мг/100 г в среднем за два года. Результаты были проанализированы с использованием однофакторного дисперсионного анализа (ANOVA) с последующим HSD-тестом Тьюки с α = 0,05 с помощью программы MINITAB (v. 19.0)

In-beam fast-timing measurements in 103,105,107Cd

Fast-timing measurements were performed recently in the region of the medium-mass 103,105,107Cd isotopes, produced in fusion evaporation reactions. Emitted gamma-rays were detected by eight HPGe and five LaBr3:Ce detectors working in coincidence. Results on new and re-evaluated half-lives are discussed within a systematic of transition rates. The $7/2_1^+$ states in 103,105,107Cd are interpreted as arising from a single-particle excitation. The half-life analysis of the $11/2_1^-$ states in 103,105,107Cd shows no change in the single-particle transition strength as a function of the neutron number